Name: Emma Kinloch
Organisation: The Francis Crick Institute
Program: Alpha

“Almost 100% delivery with no effect on cell viability”

Core Research Area:
p53, cell fate decisions, chemical biology

Which transfection or delivery methods have you used before?:
Mechanical - bead loading, cell squeezing (portal bio)

What type of cargo did you use?:
Protein

What cell type(s) did you use?:
HCT116 and H1299 (human cancer cell lines)

Was your experiment in 2D culture, 3D, or suspension?:
2d

Please briefly describe the goal of your experiment:
Delivery of GFP protein into cells at high efficiency with little effect on cell viability

How easy was the protocol to follow? (1–5 scale):
5. Very easy

How well did BubbleFect integrate into your existing workflow? (1-5 scale):
5. Very easy

What were the observed outcomes of your experiment?:
Almost 100% delivery with no effect on cell viability

Compared to your usual method, how did BubbleFect perform? (1-5 scale):
5. Much better

Would you use BubbleFect again for this application?:
Yes