BubbleFect is cargo-agnostic and has been used across a wide range of cargo types including mRNA, CRISPR/RNP, plasmid DNA, proteins and peptides, ASOs, RNA/saRNA, siRNA, and small molecules. Researchers across our community are actively using BubbleFect with additional cargo types as part of their own workflows, and our validated application range continues to grow. If you don't see your cargo type listed, get in touch — we are happy to discuss your specific application at partitionbio.co.uk/contact-us.

BubbleFect has been used across a wide and growing range of cell types including hiPSCs, HEK293, HeLa, suspension cells (FreeStyle HEK), U2OS, mouse myotubes (post-mitotic muscle fibres), Jurkat, THP-1 monocytes, HepG2 and cancer cell lines including H1299 and HCT116. This represents only a snapshot — researchers across our community are actively using BubbleFect in many additional cell types as part of their own workflows, and our validated application range continues to expand. If you are working with a cell type not listed here, get in touch — we are happy to discuss your specific application at partitionbio.co.uk/contact-us.

In head-to-head comparisons, BubbleFect matches or exceeds competitor lipofection reagents for transfection efficiency while maintaining significantly higher cell viability — above 80% compared to dips post-transfection to approximately 20% with lipofection in suspension cells. Unlike electroporation, BubbleFect requires no specialist equipment, causes minimal cell stress, and maintains a simple three-step workflow. BubbleFect is also non-lipid and non-viral, removing the toxicity and complexity associated with both approaches.

BubbleFect is designed to preserve cell health. In suspension cells, viability remains above 80% throughout the experimental time course — compared to post-transfection dips to approximately 20% with competitor lipofection reagents. In cancer cell lines, cell survival exceeds 90% following protein delivery. BubbleFect's low cytotoxicity profile makes it particularly well suited to sensitive cell types where preserving viability is critical to experimental outcomes.

Very simple. BubbleFect uses a straightforward three-step protocol — mix, incubate, deliver — with no specialist equipment required. It is designed to integrate easily into existing workflows and is reproducible across users and laboratory conditions. Full protocol documentation is available on our downloads page.

BubbleFect is stable at room temperature, requires no cold chain, and can be shipped globally without dry ice or refrigeration. This makes it significantly easier to use than many lipid-based reagents, which are temperature sensitive and require careful handling. We recommend storing in the fridge or freezer upon receipt, for longer term stability and aseptic conditions.

Yes. BubbleFect is currently available for research use only (RUO) and is not approved for clinical or therapeutic use. PartitionBio is a life science tools company and BubbleFect is intended for laboratory research applications.

BubbleFect can be ordered directly at partitionbio.co.uk, or by contacting the team via email or the enquiry form on our contact page. If the cost of an initial purchase is a barrier to getting started, please get in touch and tell us about your research — we review applications on a case by case basis and may be able to support access where there is a strong scientific fit. Contact us at partitionbio.co.uk/contact-us.

Yes. Our scientific team is available to support you with protocol questions, troubleshooting, and application-specific advice. If you are working with a challenging cell type or cargo, we are happy to discuss your experiment before you get started. Get in touch via partitionbio.co.uk/contact-us.