Product Documents
Transfection Protocols, Safety Data Sheets and Resources
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Transfection Protocols:
BubbleFect Transfection Protocol - Adherent Cells (v3)
BubbleFect Transfection Protocol - CRISPR RNP (v3)
BubbleFect Transfection Protocol – Non-Adherent Cells e.g. Jurkats
BubbleFect Large-Scale Transfection Protocol – HEK Suspension Cells
BubbleFect Transfection Protocol – Hydrophobic Small Molecule Drug Delivery
Research Use Only Licence:
Material Safety Data Sheets
Material Safety Data Sheet: BubbleFect
Creation Date: 04 July 2025
Revision Date:
Revision Number: 0
Product Name: BubbleFect
Product Code: PB-001-BF001
Material Safety Data Sheet: BubbleFect Safety data sheet for BubbleFect (PB-001-BF001), PartitionBio's non-lipid, non-viral intracellular delivery reagent. Includes handling, storage, hazard classification and first aid information for laboratory use.
Material Safety Data Sheet: Booster
Creation Date: 15 October 2025
Revision Date:
Revision Number: 0
Product Name: BubbleFect Booster
Product Code: PB-001-BF-BSTR
Material Safety Data Sheet: BubbleFect Booster Safety data sheet for BubbleFect Booster (PB-001-BF-BSTR), an add-on reagent designed to enhance BubbleFect transfection efficiency in difficult-to-transfect cell types. Includes handling, storage and hazard information.
Material Safety Data Sheet: Enhancer
Creation Date: 30 May 2026
Revision Date:
Revision Number: 0
Product Name: BubbleFect Enhancer
Product Code: PB-002-BF-ENH
Material Safety Data Sheet: BubbleFect Enhancer
Safety data sheet for BubbleFect Enhancer (PB-002-BF-ENH), an add-on reagent designed to improve BubbleFect transfection performance, particularly in difficult-to-transfect and sensitive cell types. Includes handling, storage and hazard information.
Transfection Protocols
BubbleFect Transfection Protocol – Adherent cells
Creation Date: 25 July 2025
Revision Date: 25 June 2026
Revision Number: 3
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol - Adherent cells: Step-by-step transfection protocol for BubbleFect. Includes recommended volumes, incubation times, titration guidance, and instructions for use with BubbleFect Booster and Enhancer add-ons. Suitable for delivery of mRNA, plasmid DNA, proteins, ASOs and other cargo types. Revised June 2026.
BubbleFect Transfection Protocol – CRISPR RNP
Creation Date: 27 April 2026
Revision Date: 25 June 2026
Revision Number: 3
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol - CRISPR RNP: Dedicated protocol for CRISPR RNP delivery using BubbleFect, optimised for genome editing applications in mammalian cell lines. Includes a four-step transfection table for 96-well plate format, recommended BubbleFect and RNP concentration ranges, and guidance on use with BubbleFect Enhancer. Suitable for researchers working with CRISPR-Cas9 knockout and knock-in experiments. Also includes optional instructions for nucleic acid delivery of CRISPR components and ssODN knock-in.
BubbleFect Transfection Protocol – Non-Adherent Cells e.g. Jurkats
Creation Date: 21 September 2026
Revision Date:
Revision Number: 1
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol — Non-adherent Cells e.g Jurkats.: Suspension and non-adherent cell lines such as Jurkats are notoriously difficult to transfect efficiently. This BubbleFect protocol provides both forward and reverse transfection workflows optimised for small-scale, 96-well suspension cultures, along with an optional Booster reagent for nucleic acid cargos and a recommended titration matrix to fine-tune delivery for new cell types or cargos on first use.
BubbleFect Large-Scale Transfection Protocol – HEK Suspension Cells
Creation Date: 21 September 2026
Revision Date:
Revision Number: 1
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Large-Scale Transfection Protocol - HEK Suspension Cells: Designed for scaled-up protein production, this BubbleFect protocol delivers plasmid DNA directly into shaking HEK293-F suspension cultures — from 1–5 mL deep-well formats up to 20 mL flask volumes. Benchmarked against common transfection reagents, BubbleFect achieves higher protein yield within 48 hours using less DNA input, making it a cost-effective option for suspension-based protein expression workflows.
BubbleFect Transfection Protocol – Hydrophobic Small Molecule Drug Delivery
Creation Date: 21 September 2026
Revision Date:
Revision Number: 1
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol - Hydrophobic Small Molecule Drug Delivery: BubbleFect's forward transfection protocol offers a fast, reagent-light method for delivering hydrophobic small molecules, DNA, mRNA, siRNA, ASOs, proteins, and antibodies into cultured cells. Using a straightforward Assemble–Bubbles–Cells workflow, researchers can achieve efficient cargo delivery in as little as one hour, with the protocol scalable from 96-well plates to any culture vessel and adaptable to reverse transfection or suspension cell formats.
Research Use Only Licence
Creation Date: 18 July 2025
Revision Date:
Revision Number: 0
Product Name: BubbleFect
Product Code: PB-001-BF001
Research Use Only Licence Terms and conditions governing the research use of BubbleFect (PB-001-BF001). BubbleFect is licenced for research use only and is not approved for clinical or therapeutic applications. Researchers should read this document before use.
BubbleFect Calculators
Creation Date: 28 August 2025
Revision Date:
Revision Number: V01
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Calculator (V01) Excel-based dilution calculator for BubbleFect transfection experiments. Calculates assay concentrations and working solution volumes based on user-defined inputs. Useful for setting up titration experiments and scaling protocols across different plate formats.
Creation Date: 11 December 2025
Revision Date:
Revision Number: V02
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Calculator Matrix (V02) Updated Excel calculator for BubbleFect transfection optimisation. Calculates volumes for BubbleFect, cargo, Booster and serum-free media across a titration matrix. Designed to support 5x5 or custom matrix optimisation experiments, particularly useful when working with new cell types or cargo.