BubbleFect® Protocols
Transfection Protocols
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Transfection Protocols:
BubbleFect Transfection Protocol - Adherent Cells (v3)
BubbleFect Transfection Protocol - CRISPR RNP (v3)
BubbleFect Transfection Protocol – Non-Adherent Cells e.g. Jurkats
BubbleFect Large-Scale Transfection protocol – HEK suspension cells
BubbleFect Transfection Protocol – hydrophobic small molecule drug delivery
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Transfection Protocols
BubbleFect Transfection Protocol – Adherent cells
Creation Date: 25 July 2025
Revision Date: 25 June 2026
Revision Number: 3
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol - Adherent cells: Step-by-step transfection protocol for BubbleFect. Includes recommended volumes, incubation times, titration guidance, and instructions for use with BubbleFect Booster and Enhancer add-ons. Suitable for delivery of mRNA, plasmid DNA, proteins, ASOs and other cargo types. Revised June 2026.
BubbleFect Transfection Protocol – CRISPR RNP
Creation Date: 27 April 2026
Revision Date: 25 June 2026
Revision Number: 3
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol - CRISPR RNP: Dedicated protocol for CRISPR RNP delivery using BubbleFect, optimised for genome editing applications in mammalian cell lines. Includes a four-step transfection table for 96-well plate format, recommended BubbleFect and RNP concentration ranges, and guidance on use with BubbleFect Enhancer. Suitable for researchers working with CRISPR-Cas9 knockout and knock-in experiments. Also includes optional instructions for nucleic acid delivery of CRISPR components and ssODN knock-in.
BubbleFect Transfection Protocol – Non-Adherent Cells e.g. Jurkats
Creation Date: 21 September 2026
Revision Date:
Revision Number: 1
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol — Non-adherent Cells e.g Jurkats.: Suspension and non-adherent cell lines such as Jurkats are notoriously difficult to transfect efficiently. This BubbleFect protocol provides both forward and reverse transfection workflows optimized for small-scale, 96-well suspension cultures, along with an optional Booster reagent for nucleic acid cargos and a recommended titration matrix to fine-tune delivery for new cell types or cargos on first use.
BubbleFect Large-Scale Transfection Protocol – HEK Suspension Cells
Creation Date: 21 September 2026
Revision Date:
Revision Number: 1
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Large-Scale Transfection Protocol - HEK Suspension Cells: Designed for scaled-up protein production, this BubbleFect protocol delivers plasmid DNA directly into shaking HEK293-F suspension cultures — from 1–5 mL deep-well formats up to 20 mL flask volumes. Benchmarked against common transfection reagents, BubbleFect achieves higher protein yield within 48 hours using less DNA input, making it a cost-effective option for suspension-based protein expression workflows.
BubbleFect Transfection Protocol – Hydrophobic Small Molecule Drug Delivery
Creation Date: 21 September 2026
Revision Date:
Revision Number: 1
Product Name: BubbleFect
Product Code: PB-001-BF001
BubbleFect Transfection Protocol - Hydrophobic Small Molecule Drug Delivery: BubbleFect's forward transfection protocol offers a fast, reagent-light method for delivering hydrophobic small molecules, DNA, mRNA, siRNA, ASOs, proteins, and antibodies into cultured cells. Using a straightforward Assemble–Bubbles–Cells workflow, researchers can achieve efficient cargo delivery in as little as one hour, with the protocol scalable from 96-well plates to any culture vessel and adaptable to reverse transfection or suspension cell formats.