BubbleFect Downloads
Transfection Protocols, Safety Data Sheets and Resources

QUICK LINKS

Research Use Only Licence

Research-Use-Only License

Transfection Protocols

Transfection Protocol

Creation Date: 25 July 2025
Revision Date: 25 June 2026
Revision Number: 3
Product Name: BubbleFect
Product Code: PB-001-BF001

Transfection Protocol — BubbleFect (v3) Step-by-step transfection protocol for BubbleFect. Includes recommended volumes, incubation times, titration guidance, and instructions for use with BubbleFect Booster and Enhancer add-ons. Suitable for delivery of mRNA, plasmid DNA, proteins, ASOs and other cargo types. Revised June 2026.

Transfection Protocol: CRISPR

Creation Date: 27 April 2026
Revision Date: 25 June 2026
Revision Number: 3
Product Name: BubbleFect
Product Code: PB-001-BF001

Transfection Protocol — CRISPR RNP Delivery with BubbleFect (v3) Dedicated protocol for CRISPR RNP delivery using BubbleFect, optimised for genome editing applications in mammalian cell lines. Includes a four-step transfection table for 96-well plate format, recommended BubbleFect and RNP concentration ranges, and guidance on use with BubbleFect Enhancer. Suitable for researchers working with CRISPR-Cas9 knockout and knock-in experiments. Also includes optional instructions for nucleic acid delivery of CRISPR components and ssODN knock-in.

Material Safety Data Sheets

Material Safety Data Sheet: BubbleFect

Creation Date: 04 July 2025
Revision Date:
Revision Number: 0
Product Name: BubbleFect
Product Code: PB-001-BF001

Material Safety Data Sheet: BubbleFect Safety data sheet for BubbleFect (PB-001-BF001), PartitionBio's non-lipid, non-viral intracellular delivery reagent. Includes handling, storage, hazard classification and first aid information for laboratory use.

Material Safety Data Sheet: Buffer

Creation Date: 15 October 2025
Revision Date:
Revision Number: 0
Product Name: BubbleFect Buffer
Product Code: PB-001-BF-BUF

Material Safety Data Sheet: BubbleFect Buffer Safety data sheet for BubbleFect Buffer (PB-001-BF-BUF), the accompanying buffer solution used in BubbleFect transfection workflows. Includes handling, storage and hazard information.

Material Safety Data Sheet: Booster

Creation Date: 15 October 2025
Revision Date:
Revision Number: 0
Product Name: BubbleFect Booster
Product Code: PB-001-BF-BSTR

Material Safety Data Sheet: BubbleFect Booster Safety data sheet for BubbleFect Booster (PB-001-BF-BSTR), an add-on reagent designed to enhance BubbleFect transfection efficiency in difficult-to-transfect cell types. Includes handling, storage and hazard information.

Research Use Only Licence

BubbleFect Research Use Only licence document, PartitionBio

Research-Use-Only License

Creation Date: 18 July 2025
Revision Date:
Revision Number: 0
Product Name: BubbleFect
Product Code: PB-001-BF001

Research Use Only Licence Terms and conditions governing the research use of BubbleFect (PB-001-BF001). BubbleFect is licensed for research use only and is not approved for clinical or therapeutic applications. Researchers should read this document before use.

BubbleFect Calculators

BubbleFect dilution calculator spreadsheet showing assay concentrations and working solution volumes

BubbleFect Calculator

Creation Date: 28 August 2025
Revision Date:
Revision Number: V01
Product Name: BubbleFect
Product Code: PB-001-BF001

BubbleFect Calculator (V01) Excel-based dilution calculator for BubbleFect transfection experiments. Calculates assay concentrations and working solution volumes based on user-defined inputs. Useful for setting up titration experiments and scaling protocols across different plate formats.

BubbleFect Calculator Matrix

Creation Date: 11 December 2025
Revision Date:
Revision Number: V02
Product Name: BubbleFect
Product Code: PB-001-BF001

BubbleFect Calculator Matrix (V02) Updated Excel calculator for BubbleFect transfection optimisation. Calculates volumes for BubbleFect, cargo, Booster and serum-free media across a titration matrix. Designed to support 5x5 or custom matrix optimisation experiments, particularly useful when working with new cell types or cargo.