Protein Delivery
Protein Delivery Without Compromise
High-efficiency delivery for functional protein studies
Delivering proteins into living cells underpins modern life science research, from functional studies and assay development to therapeutic screening and intracellular mechanistic investigations. Yet achieving consistent, efficient delivery without harming cells remains a challenge.
BubbleFect is a next-generation cell delivery technology designed to simplify and optimise protein delivery workflows across a wide range of cell types.
Traditional protein delivery methods often force a compromise: aggressive chemistries drive uptake but damage cells, while gentler approaches rely on inefficient or variable endocytic pathways. BubbleFect overcomes these limitations through a controlled, peptide-driven delivery mechanism that stabilises proteins during transfer and promotes consistent cellular entry with minimal membrane disruption.
BubbleFect forms transient, tunable delivery assemblies that associate non-covalently with purified proteins and protein complexes. These assemblies protect cargo from aggregation and degradation, helping preserve native structure and activity during delivery. By optimising the size, stability, and concentration of these assemblies, BubbleFect enables reproducible uptake across a range of cell types without triggering the stress responses commonly associated with lipid- or polymer-based systems.
As a result, BubbleFect supports scalable, cell-friendly protein delivery that translates directly into functional intracellular activity. Whether delivering enzymes, antibodies, reporter proteins, or engineered variants, BubbleFect provides a reliable and predictable route from purified protein to measurable biological function inside the cell.
A Better Way to Deliver Proteins
A new phase in cell delivery
From
£80
Seamless cell delivery
as easy as A B C
-
A – Assemble
Mix your cargo with BubbleFect in water – done.
Simply combine your nucleic acid or protein cargo with BubbleFect. Mix by pipetting or vortexing.
💡 First-time users can run a quick titration to optimise for their system. -
B – Bubbles
Add serum-free medium to instantly create Bubbles.
Pipette in serum-free medium – Bubbles form on contact. The solution turns cloudy and is ready to use, no incubation needed. -
C – Cells
Transfer Bubbles onto your cells – that’s it.
Add an equal volume of Bubbles to cells. Incubate for 1 hour. Optional: wash cells in rich medium and proceed with your standard assay protocol.
Why it works
BubbleFect integrates into existing protocols with no disruption. It’s fast and requires little optimisation. Making high-efficiency delivery accessible for any laboratory.
FULL PROTOCOL
Why BubbleFect for Protein Delivery?
Optimised delivery efficiency
BubbleFect enables robust intracellular delivery, supporting strong functional activity while maintaining cell health.
Cell-friendly approach
Using LLPS-driven delivery systems, PartitionBio supports precise, non-viral intracellular delivery of proteins.
Reproducible results
Designed for consistency, BubbleFect reduces variability between experiments, supporting reliable comparisons across proteins, conditions, and batches.
Scalable from discovery to production
From small-scale assays to larger culture formats, BubbleFect integrates into common laboratory workflows for straightforward scale-up.
A new phase in cell delivery
From
£80
Built for Modern Protein Delivery Workflows
BubbleFect integrates easily into existing experimental pipelines, supporting:
Delivery of purified proteins for functional studies
Reporter proteins for pathway analysis
Screening of protein variants
Proof-of-concept experiments prior to more complex assays
With optional BubbleFect Booster and Buffer, researchers can refine delivery conditions to maximise efficiency while maintaining experimental control.
Designed for Data-Driven Optimisation
BubbleFect is supported by tools like the BubbleFect Matrix Calculator, enabling systematic optimisation of delivery conditions. This structured approach replaces trial-and-error with precise titration experiments, helping teams balance efficiency, protein activity, and cell viability.
Unlock More from Your Protein Delivery Experiments
Protein delivery shouldn’t be a bottleneck. With BubbleFect, researchers gain a flexible, efficient platform designed for reproducible, high-quality delivery across diverse applications.
Explore BubbleFect for protein delivery and take control of your intracellular experiments.
Supporting Data: See the Science
eGFP mRNA expression in non-dividing terminally differentiated muscle myotube cells
In comparative studies, BubbleFect demonstrated a significant improvement in transfection efficiency, increasing the reporter protein level to ten times higher than that achieved by a leading lipid-based competitor under identical conditions. This increase in yield reflects the higher payload delivery that is possible with BubbleFect, with minimal toxicity or disruption to cell health. The result is superior performance, especially in applications requiring high expression levels, broader cell coverage, or sensitive cell types. BubbleFect’s efficient, reagent-only workflow also makes it easier to reproduce these results across labs and experiments.
Delivering Antibodies Inside Cells to Unlock New Frontiers in Research and Therapeutics
BubbleFect enables efficient intracellular delivery of targeting antibodies, expanding the potential of antibody-based research and therapeutics beyond surface markers and secreted proteins. This opens the door to entirely new applications, including functional proteomics and subcellular targeting, powered by data from resources like the Human Protein Atlas. With BubbleFect, intracellular delivery of charge-neutral cargos such as proteins of any class is no longer a barrier — it's a breakthrough.
A new phase in cell delivery
From
